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. 2020 Dec 8;21(24):9344. doi: 10.3390/ijms21249344

Figure 2.

Figure 2

Ang-(1-7) decreased the LPS-induced LC3II/LC3I ratio via the Mas receptor in muscles from mice. C57BL/6J wild-type or Mas KO male mice were treated with the vehicle, LPS, Ang-(1-7), or LPS + Ang-(1-7) for 18 h. At the end of the experiment, the mice were sacrificed, and the muscle was excised and homogenized to evaluate LC3I and LC3II protein levels through Western blot analysis in DFG (A,C), TA (E,G), and GA (I,K). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels are shown as loading control. Molecular weight markers are depicted in kDa. The quantitative analysis of the experiments is shown for DFG (B,D), TA (F,H), and GA (J,L). The results were represented as LC3II/LC3I ratio and expressed as the mean ± S.E. (the fold of change relative to the vehicle group). Nine animals per group were used in these experiments (*, p < 0.05 vs. vehicle. #, p < 0.05 vs. LPS). Plus (+) and minus (−) symbols indicated in the figures or graphics mean presence or absence of treatment.