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. 2020 Dec 8;21(24):9344. doi: 10.3390/ijms21249344

Figure 5.

Figure 5

Ang-(1-7) inhibited the LPS-induced p38, JNK, and BCL-2 phosphorylation in C2C12 cells. C2C12 cells were pre-incubated in the absence or presence of Ang-(1-7) for 30 min (100 nM). Then, the cells were incubated without or with LPS (500 ng/mL) for 30 min to analyze p38 (A) or JNK (C) phosphorylation and for 4 h for BCL-2 phosphorylation (E) by Western blot. The levels of GAPDH are shown as loading control. Molecular weights are shown in kDa. (B,D,F) show the quantitative analysis of the experiments represented in (A,C,E), respectively. The protein levels of phospho-p38, phospho-JNK, and phospho-BCL-2 were normalized to GAPDH and expressed as the mean ± S.E. (the fold of change relative to the control). Three or four independent experiments were performed (*, p < 0.05 vs. vehicle. #, p < 0.05 vs. LPS). Plus (+) and minus (−) symbols indicated in the figures or graphics mean presence or absence of treatment.