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. 2020 Dec 8;21(24):9344. doi: 10.3390/ijms21249344

Figure 6.

Figure 6

Ang-(1-7) prevented the LPS-induced disassembly of Beclin1/BCL-2 complex C2C12 cells. (A) C2C12 cells were incubated with LPS (500 ng/mL) at different times from 0 to 4 h. The interaction between Beclin1 and BCL-2 was analyzed using immunoprecipitation with anti-BCL-2. From the eluate, the Beclin1 and BCL-2 protein levels were detected by Western blot. The protein levels of GAPDH are shown as loading control. Molecular weights are shown in kDa. (B) Quantification of three independent experiments represented in (A). The Beclin1/BCL-2 ratio from the eluate was normalized to GAPDH and expressed as the mean ± S.E. (fold of change relative to time 0. *, p < 0.05 vs. vehicle). (C) C2C12 cells were pre-incubated in the absence or presence of Ang-(1-7) (100 nM) for 30 min. Then, the cells were incubated without or with LPS (500 ng/mL) for 4 h. The interaction between Beclin1 and BCL-2 was determined as in (A). (D) Quantification of three independent experiments represented in (C). The Beclin1/BCL-2 ratio from the eluate was normalized to GAPDH and expressed as the mean ± S.E. (the fold of change relative to the control. *, p < 0.05 vs. vehicle. #, p < 0.05 vs. LPS). Plus (+) and minus (−) symbols indicated in the figures or graphics mean presence or absence of treatment.