Figure 4.

The qPCR results and the determination of beta-amyloid protein. After 28 days of culturing, Tuj1 and Map2 significantly increased. But there was no significant difference in expression of neuronal markers Tuj1 and Map2 between iPS-22-1 and iPS-15-5 cell (a, b). However, the expression of cortical neuron markers SATB2 and TBR1 of iPS-15-5 was higher than that of iPS-22-1 (c, d). ^∗P < 0.05, ^∗∗P > 0.05, and ^∗∗∗P < 0.01. n = 3. The contents of Aβ 1-40 and Aβ 1-42 in adherent culture of neurons in AD patients had been higher than those in normal people (e, f). ^∗P < 0.05, ^∗∗P < 0.01. n = 3. The content of AβO in adherent culture solution of neurons in AD patients had been higher than that of normal people (g). ^∗P < 0.05. n = 3.