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. 2020 Dec 26;34(2):108630. doi: 10.1016/j.celrep.2020.108630

Figure 3.

Figure 3

Biophysics and Structure of the S-GSAS/D614G Ectodomain

(A) (Left) SEC elution profile on a Superose 6 10/300 column of the S-GSAS/D614G (blue) ectodomain. Fractions isolated for further characterization are indicated by vertical red dotted lines. Elution volumes of standard at 669 and 44 kDa are labeled for reference. (Middle) SDS-PAGE of the SEC purified ectodomain. (right) Differential scanning fluorimetry (DSF) of S-GSAS/D614G (blue). Thermal melting inflection points (Ti) are indicated on the first derivative graph and reported in the table below from a triplicate.

(B) Representative NSEM micrograph of S-GSAS/D614G and 2D class averages (related to Data S2).

(C) Side view of the cryo-EM reconstruction of the 1-RBD-up (EMD: 22826) and the 3-RBD-down (EMD: 22825) states of the S-GSAS/D614G ectodomain colored by chain. The up positioned RBD in the map is identified by an asterisk (related to Table S1 and Data S2).

(D) (Left) Top view of the 1-RBD-up S trimer shown in (C). (Right) Subpopulations obtained by further classification (EMD: 22835, 22836, 22837, and 22838) (related to Figure S2 and Data S3).

(E) (Left) Top view of the 3-RBD-down S trimer shown in (C). (Right) Subpopulations obtained by further classification (EMD: 22831, 22832, 22833, and 22834) (related to Figure S2 and Data S3).