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. 2020 Dec 26;34(2):108630. doi: 10.1016/j.celrep.2020.108630

Figure 7.

Figure 7

Structure and Antigenicity of the Furin-Cleaved S-RRAR/D614G Ectodomain

(A) Side view of the cryo-EM reconstruction of the 1-RBD-up (EMD: 22824) and the 3-RBD-down (EMD: 22823) states of the furin-cleaved S-RRAR/D614G ectodomain colored by chain. The up positioned RBD in the map is identified by an asterisk. The NTDs in the asymmetric 1-RBD-up structure are labeled (related to Table S1 and Data S5).

(B) Binding of ACE2 receptor ectodomain (RBD-directed), CR3022 (RBD-directed neutralizing antibody), 2G12 (S2-directed), Ab712199 (RBD-directed neutralizing antibody), and Ab511584 (S2-directed non-neutralizing antibody) to S-GSAS/D614G (in blue) and the furin-cleaved S-RRAR/D614G ectodomain (in green) measured by ELISA. The assay format was the same as in Figure 2D.

(C) Overlay of the individual protomers in the 1-RBD-up structure (PDB: 7KDJ) and a protomer in the C3 symmetric 3-down-RBD structure (PDB: 7KDI) shown in (A). RBD-up chain with the S1 subunit colored by domain and the S2 subunit colored gray. RBD is colored red, NTD green, SD1 dark blue, SD2 orange, and the linker between the NTD and RBD cyan. The down RBDs are colored salmon, and the SD1 domains from the down RBD chains are colored light blue. The linker between the NTD and RBD in the down RBD chains is colored deep teal. Insets show zoomed-in views of individual domains similar to the depiction in Figure 4D.

(D) (Left) The protomers of the 1-RBD-up structure of the furin-cleaved S-RRAR/D614G ectodomain superimposed using residues 908–1,035 and colored by the color of their NTD as depicted in (A). Zoomed-in views show region of the SD2 domain proximal to the NTD.