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. 2020 Dec 22;1(9):100157. doi: 10.1016/j.xcrm.2020.100157

Figure 2.

Figure 2

cTfh Are Activated in P. falciparum Infection

(A) cTfh (PD1+CXCR5+CD4) T cells were assessed by flow cytometry at days 0, 8, and 14/15, and EOS.

(B) cTfh cells as a frequency of total CD4 T cells after infection.

(C) Representative flow cytometry gating of activated (ICOS+) cTfh cells following infection.

(D) Activated cTfh (ICOS+) cells as a frequency of CD4 T cells.

(E) Representative histograms of CD38 expression on activated (ICOS+) and non-activated (ICOS−) cTfh cells.

(F) CD38 positivity as a frequency of activated ICOS+ cTfh cells. Flow cytometry-based assays were performed once on whole-blood samples tested in singles.

Data are from 40 individuals at days 0, 8, and 14/15 and 37 individuals at EOS. (A), (C), and (E) are representative data from a single individual. For (B), (D), and (F), paired Wilcoxon signed-rank tests compared to day 0 are indicated. Boxplots are Tukey style, with median and interquartile range (IQR). Whiskers are to data point no further than 1.5 × IQR; dots are outlying points. Paired data are joined by lines. See also Figures S1 and S2.