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. 2020 Dec 22;1(9):100157. doi: 10.1016/j.xcrm.2020.100157

Figure 4.

Figure 4

Memory Phenotypes of Tfh Subsets during Infection

(A) cTfh (PD1+CXCR5+CD4) cells were differentiated into memory phenotypes based on CD45RA and CCR7 expression into central memory (CM CD45RA−CCR7+), effector memory (EM CD45RA−CCR7−), terminal differentiated effector memory (TEMRA CD45RA+CCR7−), and naive (CD45RA+CCR7+) phenotypes.

(B) cTfh subsets (Th1, Th2, Th17, based on CXCR3 and CCR6 expression) had different distributions of memory phenotypes.

(C) Mean memory phenotypes across subsets at day 0, before inoculation.

(D) Proportion of CM and EM phenotypes within cTfh subsets after infection. Flow cytometry-based assays were performed once on cryopreserved PBMC samples tested in singles.

Data are from 6 individual study participants from days 0, 8, and EOS (cryopreserved PBMC day 14/15 samples were not available). (A) and (B) are representative data from 1 individual. (C) is the median of each memory phenotype in each subset. (D) Boxplots are Tukey style, with median and IQR. Whiskers are to data point no further than 1.5 × IQR, dots are outlying points. Paired data are joined by lines. Wilcoxon signed-rank test compared to day 0 are indicated.