| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat anti-human IgG HRP-conjugate | Thermo Fisher Scientific | Cat#62-8420; RRID:AB_2533962 |
| Mouse anti-human IgG1 subclass (clone HP6069) | Thermo Fisher Scientific | Cat# A-10630; RRID:AB_2534049 |
| Mouse anti-human IgG2 subclass (clone HP6002) | Thermo Fisher Scientific | Cat#05-3500; RRID:AB_2532258 |
| Mouse anti-human IgG3 subclass (clone HP6050) | Thermo Fisher Scientific | Cat#05-3600; RRID:AB_2532261 |
| Mouse anti-human IgG4 subclass (clone HP6025) | Thermo Fisher Scientific | Cat#A10651; RRID:AB_2534053 |
| Mouse anti-human IgM (clone HP6083) | Thermo Fisher Scientific | Cat#054900; RRID:AB_2532927 |
| Goat anti-mouse IgG HRP-conjugate | Merck Millipore | Cat#AP308P; RRID:AB_92635 |
| Goat anti-rabbit IgG HRP-conjugate | Abcam | Cat#AB97051; RRID:AB_10679369 |
| Rabbit anti-human C1q | In-house | Boyle et al.27 |
| Strepavidin HRP-conjugate | Thermo Fisher Scientific | Cat#21130 |
| IBSM whole blood panel | ||
| Mouse anti-human CD20 BUV395 (Clone 2H7) | BD Biosciences | Cat#563782; RRID:AB_2744325 |
| Rat anti-human CXCR5 BV421 (Clone RF8B2) | BD Biosciences | Cat#562747; RRID:AB_2737766 |
| Mouse anti-human CD4 V500 (Clone RPA-T4) | BD Biosciences | Cat#560768; RRID:AB_1937323 |
| Mouse anti-human CCR6 BV650 (Clone 11A9) | BD Biosciences | Cat#563922; RRID:AB_2738488 |
| Mouse anti-human CD38 BV786 (Clone HIT2) | BD Biosciences | Cat#563964; RRID:AB_2738515 |
| Mouse anti-human CXCR3 APC (Clone 1C6) | BD Biosciences | Cat#550967; RRID:AB_398481 |
| Mouse anti-human CD27 APC-R700 (Clone M-T271) | BD Biosciences | Cat#565116; RRID:AB_2739074 |
| Mouse anti-human CD8 APC-Cy7 (Clone SK1) | BD Biosciences | Cat#557834; RRID:AB_396892 |
| Mouse anti-human CD19 FITC (Clone HIB19) | BD Biosciences | Cat#55412 |
| Mouse anti-human CD45 PerCP-Cy5.5 (Clone 2D1) | BD Biosciences | Cat#340953; RRID:AB_400194 |
| Mouse anti-human ICOS PE (Clone DX29) | BD Biosciences | Cat#557802; RRID:AB_396878 |
| Mouse anti-human CD3 PE-CF594 (Clone UCHT1) | BD Biosciences | Cat#562280; RRID:AB_11153674 |
| Mouse anti-human PD-1 PE-Cy7 (Clone EH12.1) | BD Biosciences | Cat#561272; RRID:AB_10611585 |
| IBSM PBMC panel | ||
| Mouse anti-human CD45RA BUV563 (Clone HI100) | BD Biosciences | Cat#612926; RRID:AB_2870211 |
| Mouse anti-human CD4 BUV737 (Clone OKT4) | BD Biosciences | Cat#612748; RRID:AB_2870079 |
| Rat anti-human CXCR5 BV421 (Clone RF8B2) | BD Biosciences | Cat#562747; RRID:AB_2737766 |
| Mouse anti-human CCR6 BV650 (Clone 11A9) | BD Biosciences | Cat#563922; RRID:AB_2738488 |
| Mouse anti-human PD-1 APC(Clone EH12.1) | Biolegend | Cat#329908; RRID:AB_940475 |
| Mouse anti-human CD3 FITC (Clone UCHT1) | BD Biosciences | Cat#555332; RRID:AB_395739 |
| Mouse anti-human CCR7 PerCP-Cy5.5 (Clone 150503) | BD Biosciences | Cat#561144; RRID:AB_10562553 |
| Mouse anti-human CXCR3 PE-CF594 (Clone 1C6) | BD Biosciences | Cat#562451; RRID:AB_11153118 |
| Mouse anti-human Ki67 BUV395 (B56) | BD Biosciences | Cat#564071; RRID:AB_2738577 |
| Mouse anti-human HLA-DR APC-H7 (G46-6) | BD Biosciences | Cat#561358; RRID:AB_10611876 |
| Live Dead Zombie Aqua | Biolegend | Cat#423102 |
| In vitro pRBC stim panel- control PBMC | ||
| Mouse anti-human CXCR3 Pacific Blue (Clone G025H7) | Biolegend | Cat#353724; RRID:AB_2561442 |
| Mouse anti-human CXCR5 BV711 (Clone 12G5) | Biolegend | Cat#356934; RRID:AB_2629526 |
| Mouse anti-human CCR6 BV650 (Clone 11A9) | BD Biosciences | Cat#563922; RRID:AB_2738488 |
| Mouse anti-human CD3 AF532 (Clone UHCT1) | Invitrogen | Cat#58003842 |
| Mouse anti-human CD4 PerCP-Cy5.5 (Clone OKT4) | Biolegend | Cat#317428; RRID:AB_1186122 |
| Mouse anti-human PD-1 PE-Cy7 (Clone EH12.1) | BD Biosciences | Cat#561272; RRID:AB_10611585 |
| Hamster anti-human ICOS APC-Cy7 (Clone C398.4A) | Biolegend | Cat#313530; RRID:AB_2566128 |
| Mouse anti-human Ki67 FITC (Clone B56) | BD Biosciences | Cat#584071 |
| Mouse anti-human CD38 BV480 (Clone HIT2) | BD Biosciences | Cat#566137; RRID:AB_2739535 |
| Live Dead Zombie NIR | Biolegend | Cat#423106 |
| Biological Samples | ||
| Human serum samples | QIMR-Berghofer | McCarthy et al.39 |
| Human PBMCs | QIMR-Berghofer | McCarthy et al.39 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Merozoite Surface Protein 2 (MSP2) | HEK293 Freestyle cells | McCarthy et al.39 |
| FcγR binding - rsFcγRIIa H131 ectodomain dimer | HEK293 Freestyle cells | Wines et al.34 |
| FcγR binding - rsFcγRIIIa V158 ectodomain dimer | HEK293 Freestyle cells | Wines et al.34 |
| Purified complement C1q | Merck Millipore | Cat#204876 |
| Experimental Models: Organisms/Strains | ||
| Humans inoculated with P. falciparum 3D7-parasitised erythrocytes | QIMR-Berghofer | McCarthy et al.39 |
| Software and Algorithms | ||
| R Studio | Version 1.1.456 | https://rstudio.com/ |
| GraphPad Prism | Version 7 | https://www.graphpad.com/scientific-software/prism/ |
| Flowjo | Version 10.6 | https://www.flowjo.com/ |
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