REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat Anti-Human IgG (Fc specific), highly cross adsorbed-Alkaline Phosphatase	Sigma Millipore	Cat. #SAB3701277
4G2 (anti-flavivirus E)	Stephen Whitehead	Cat. #ATCC HB-112
anti-human CD3 (UCHT1) FITC-conjugated	BioLegend	Cat. #300406
anti-human CD3 (UCHT1) Pacific Blue-conjugated	BioLegend	Cat. #300431
anti-human CD3 (UCHT1) AlexaFlour700-conjugated	BD Biosciences	Cat. #557943
anti-human CD4 (OKT4, BV510-conjugated	BioLegend	Cat. #317444
anti-human CD14 (MΦPg) BUV395-conjugated	BD Biosciences	Cat. # 563561
anti-human CD14 (HCD14) Pacific Blue-conjugated	BioLegend	Cat. #325616
anti-human CD19 (HIB19) PE-Dazzle 594-conjugated	BioLegend	Cat. #302252
anti-human CD19 (SJ25C1) BUV395	BD Biosciences	Cat. # 563549
anti-human CD20 (2H7) PE-Cy7-conjugated	BioLegend	Cat. # 302311
anti-human CD22 microbeads	Miltenyi	Cat. # 130-046-401
anti-human CD27 (O323) BrilliantViolet510-conjugated	BioLegend	Cat. #302835
anti-human CD27 (O323) PE-Cy7-conjugated	BioLegend	Cat. #302838
anti-human CD38 (HIT2) AlexaFluor 647-conjugated	BioLegend	Cat. #303514
anti-human IgM (MHM-88, PerCP-Cy5.5)	BioLegend	Cat. #314512
anti-human IFN-γ (4S.B3) FITC	eBioscience	Cat. #11-7319-41
Bacterial and Virus Strains
Dengue virus serotype 1 (West Pacific 74) WHO reference strain	Stephen Whitehead, National Institute of Allergy and Infectious Disease (NIAID)	GenBank AY145121
Dengue virus serotype 2 (New Guinea C)	Stephen Whitehead, NIAID	GenBank AF038403.1
Dengue virus serotype 2 (strain S-16803) WHO reference strain	Aravinda De Silva, University of North Carolina-Chapel Hill (UNC)	GenBank GU289914
Dengue virus serotype 3 (Sleman/78)	Stephen Whitehead, NIAID	GenBank AY656169
Dengue virus serotype 3 (CH53489) WHO reference strain	Aravinda De Silva, UNC	GenBank DQ863638
Dengue virus serotype 4 Dominica/81	Stephen Whitehead, NIAID	GenBank AY648301
Dengue virus serotype 4 (TVP-376) WHO reference strain	Aravinda De Silva, UNC	GenBank KC963424
Biological Samples
Human Serum	University of Vermont Vaccine Testing Center and Johns Hopkins Center for Immunization Research	Clinicaltrials.gov identifiers: NCT01072786, NCT02021968
Peripheral blood mononuclear cells (PBMC)	University of Vermont Vaccine Testing Center and Johns Hopkins Center for Immunization Research	Clinicaltrials.gov identifiers: NCT01072786, NCT02021968
Chemicals, Peptides, and Recombinant Proteins
recombinant human IL-21	Peprotech	Cat. #200-21
p-Nitrophenyl phosphate	Sigma	Cat. #N2770
Retronectin	Takara	Cat. #T202
Polybead Microspheres	Polysciences Inc,	Cat. #17135-5
Dynabeads M-280, Tosylactivated	ThermoFisher	Cat. #14204
Ionomycin	Millipore Sigma	Cat. # I3909-1ML; CAS: 56092-81-0
Phorbol 12-myristate 13-acetate	Millipore Sigma	Cat. #P8139; CAS: 16561-29-8
GolgiPlug	BD Biosciences	Cat. #555029
DENV-specific MHC class II peptide megapool	A&A, San Diego, CA	http://www.iedb.org, 33
Dimethyl sulfoxide	Millipore Sigma	Cat. # D2650; CAS: 67-68-5
LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation	ThermoFisher	Cat. #L34962
4’,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI)	ThermoFisher	Cat. #D1306; CAS: 28718-90-3
Experimental Models: Cell Lines
African green monkey kidney cells (Vero-81)	Stephen Whitehead, NIAID	RRID:CVC_0059
Stable CD40L-L cells	Hergen Spits, U. Amsterdam	N/A
Recombinant DNA
LZRS-BCL6-T2A-BCLXL-IRES-GFP	Diehl Lab	N/A
Software and Algorithms
Prism 8.4.3	Graphpad, Inc.	N/A
R 3.6.1	R Group	http://cran.wustl.edu/bin/macosx/
FlowJo 10	Treestar	N/A