Figure 2.

hiPSC-ECs Carrying the SERPINE1-FOSB Translocation Show Increased FOSB Expression

(A) Schematic overview of the differentiation protocol and purification of ECs from hiPSCs.

(B) Bright-field images showing typical EC morphology of hiPSC-ECs. Scale bar represents 500 μm.

(C) Fluorescence-activated cell sorting (FACS) analysis of EC marker expression on isolated ECs at passage 3 (P3) from hiPSC-ECs^WT (black-filled histogram) and hiPSC-ECs^{SERPINE1-FOSB (D3)} (red-filled histogram), and relevant isotype control (gray-filled histogram).

(D) Quantification of normalized relative surface expression levels (MFI) of VEC, VEGFR2, VEGFR3, CD31, CD34, and CD105. n = 3 (biological replicates, 3 independent batches of hiPSC-ECs). Error bars are SDs, ∗p < 0.005.

(E) Real-time qPCR analysis of FOSB expression in hiPSCs^WT, hiPSCs^{SERPINE1-FOSB (D3)}, hiPSC-ECs^WT, and hiPSC-ECs^{SERPINE1-FOSB (D3)} normalized to the housekeeping gene HPRT1 (×1,000). n = 3 (biological replicates, 3 independent batches of hiPSC-ECs). Error bars represent means ± SDs.

(F) Western blot of FOSB expression in hiPSC-ECs^WT and hiPSC-ECs^{SERPINE1-FOSB (D3)}. Short and long exposure of the gel is shown. USP7 was used as a housekeeping control.