Skip to main content
. 2020 Dec 22;1(9):100153. doi: 10.1016/j.xcrm.2020.100153

Figure 4.

Figure 4

Functional Assessment of hiPSC-ECs Carrying the SERPINE1-FOSB Translocation

(A) Analysis of hiPSC-ECsWT and hiPSC-ECsSERPINE1-FOSB (D3) proliferation rates when cultured in basal endothelial cell growth medium supplemented with 1% PPS (1%), 1% PPS supplemented with 50 ng/mL VEGF (1% VE), or complete EC growth medium (full) for 24 h. Proliferation was determined by using a Presto Blue assay. n = 3 (biological replicates, 3 independent batches of hiPSC-ECs). Error bars are shown as SDs; ∗p < 0.0001 and ∗∗p < 0.0005.

(B) Representative images of Matrigel tube formation assay using hiPSC-ECsWT and hiPSC-ECsSERPINE1-FOSB (D3) at the 48-h time point. Scale bar represents 500 μm. The right panel shows the quantification of the number of junctions and meshes. Error bars are SDs; ∗p < 0.005 and ∗∗p < 0.001.

(C) Representative absolute resistance of the EC monolayer in complete EC growth medium. N = 6 (2 independent experiments with 3 batches of hiPSC-ECs). Errors bars are shown as means ± SDs.

(D) Normalized resistance (4 kHz) of the EC monolayer in complete EC growth medium. N = 6 (2 independent experiments with 3 batches of hiPSC-ECs). Error bars are shown as means ± SDs; ∗∗∗p < 0.001.

(E) Representative immunofluorescent images of CD31 and ZO1 to analyze the cell tight junctions. Merged images show CD31 in red, ZO1 in green, and DAPI in blue. The right panels show further enlarged areas selected from the shown images (dashed squares). Scale bar represents 50 μm.