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Generation of Barcoded HIV Plasmid Library
(A) Location of barcode upstream of the HA tag in the genome of HIV strain NL-HA.
(B) Example of individual 21 nucleotide “barcode” sequences in which each 3rd nucleotide is constrained to a thymine.
(C) Complexity of barcode region as quantified by HiSeq sequencing, showing ~18,000 barcodes with relatively equal distribution.
(D) Average pairwise distance between barcodes is ~11 bp.
