Figure 2.

In Vitro Analysis of Barcoded HIV Swarm

(A) Transfection with NLHABC plasmid produces similar HIV virion concentrations to transfection with the parental NL-HA plasmid as quantified by HIV p24 protein levels. n = 3 transfections (biological replicates) for NL-HA and 9 transfections (biological replicates) for NLHABC. Error bars, mean ± 1 SD.

(B) Comparison between barcode occurrence in original plasmid DNA and resultant virion-associated viral RNA after HIV production in 293FT cells (Spearman correlation ρ= 0.99).

(C) Infection of GHOST cells for 2 days shows similar infectivity between NL-HA and NLHABC in a cell line model (n = 3 independent experiments).

(D) Infection of co-stimulated primary human PBMCs (n = 3 biological replicate infections per condition using cells from different human donors) for 3 days shows similar in vitro replication rate between NL-HA and NLHABC.

Statistical comparisons in (A), (C), and (D) performed with unpaired, 2-sided t test.

See also Figure S1.