Figure 2.

Single-Cell RNA-Seq Analyses of Tumor Slices from 8 MSS CRLM

(A) K-means clustering identifies 7 categories of cells from a total of 7,580 cells derived from all 8 cases in all treatment groups. Three tumor slices were used for single-cell dissociation in each treatment group for each of the 8 cases. The inferred cell type is shown in color on a t-SNE projection.

(B) Representative marker gene expressions are shown for cell clusters 2–7.

(C and D) Heatmaps showing cell types based on mRNA expression of selected genes (C) and cell surface markers (D).

(E) Stem-like cluster 5 is further divided into 2 subtypes, 5.1 and 5.2, with GSEA showing distinct differences in pathway utilization.

(F) Uniform manifold approximation and projection for dimension reduction (UMAP) plot of clusters 5.1 and 5.2 highlighting cells in G1, G2/M, and S phases of the cell cycle based on their transcript expression.

(G) RNA velocities overlaid on UMAP of cluster 5 showing the trajectories of clusters 5.2 (less proliferative) and 5.1 (higher proliferative) cells.