Figure 3.

Histopathological Characterization and Gene-Expression Profiling of Healthy and Tumor Organoids

(A) Histological images of organoids derived from mouse lung, kidney, liver, and human lung (P1), liver (P2), and gastric (P25) tumors and the comparison with their parental tissues/tumors. Scale bar, 200 μm.

(B–D) Immunofluorescence staining for (B) E-cadherin, (C) EpCAM, and (D) vimentin of the organoids in (A). Scale bar, 200 μm.

(E and F) Heatmap of gene expression of the organoids and their parental (E) tissues and (F) tumors by RNA-seq. It profiles (E) the organ-associated genes and chemotherapy-related genes and (F) the cancer genes and chemotherapy-related genes.

(G) Venn diagram showing ∼97% mutational (SNV) overlap between the P31 tumor organoids and the parental tumor tissue.

(H) Overview of the oncogene mutations detected in the P31 tumor organoids and the parental tumor tissue.

See also Figure S3.