REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-E-cadherin	Abcam	Cat# ab76055; RRID:AB_1310159
Rabbit monoclonal anti-EpCAM	Abcam	Cat# ab71916; RRID:AB_1603782
Rabbit monoclonal anti-Vimentin	Abcam	Cat# ab92547; RRID:AB_10562134
Rabbit monoclonal anti-CD3	Abcam	Cat# ab135372; RRID:AB_2884903
Anti-Mouse IgG H&L (Alexa Fluor® 594)	Abcam	Cat# ab150116; RRID:AB_2650601
Anti-Rabbit IgG H&L (Alexa Fluor® 594)	Abcam	Cat# ab150080; RRID:AB_2650602
Biological Samples
Human tumor tissue samples	This study	NA
Chemicals, Peptides, and Recombinant Proteins
DMEM/F12	Thermo Fisher Scientific	Cat# 11320033
Fetal bovine serum	invigentech	Cat# A6903FBS
GlutaMAX Supplement	Thermo Fisher Scientific	Cat# 35050061
HEPES	Thermo Fisher Scientific	Cat# 15630080
Penicillin-Streptomycin	Thermo Fisher Scientific	Cat# 15140122
B-27 Supplement	Thermo Fisher Scientific	Cat# 17504044
red blood cell lysis buffer	Roche	Cat# 11814389001
Nicotinamide	Sigma-Aldrich	Cat# N0636
N-Acetylcysteine	Sigma-Aldrich	Cat# A9165
Noggin (Human)	MCE	Cat# HY-P7051A
Noggin (Mouse)	MCE	Cat# HY-P7086
R-spondin 1	MCE	Cat# HY-P7114
SB431542	MCE	Cat# HY-10431
CHIR99021	MCE	Cat# HY-10182
FGF4	MCE	Cat# HY-P7014
FGF-basic(Human)	MCE	Cat# HY-P7004
FGF-basic(Mouse)	MCE	Cat# HY-P7066
FibrOut	Chi scientific	Cat# 4-21547, 4-21565, 4-21502, 4-20520
Y-27632	Abmole	Cat# M1817
EGF	Peprotech	Cat# AF-100-15
AQIX ®	AQIX LTD	NA
cyclophosphamide	MCE	Cat# HY-L025
ifosfamide	MCE	Cat# HY-L025
methotrexate	MCE	Cat# HY-L025
cytarabine	MCE	Cat# HY-L025
gemcitabine	MCE	Cat# HY-L025
epirubicin	MCE	Cat# HY-L025
daunorubicin	MCE	Cat# HY-L025
mitomycin C	MCE	Cat# HY-L025
actinomycin D	MCE	Cat# HY-L025
aclacinomycin	MCE	Cat# HY-L025
rapamycin	MCE	Cat# HY-L025
paclitaxel	MCE	Cat# HY-L025
vincristine	MCE	Cat# HY-L025
homoharringtonine	MCE	Cat# HY-L025
carboplatin	MCE	Cat# HY-L025
cisplatin	MCE	Cat# HY-L025
oxaliplatin	MCE	Cat# HY-L025
rituximab	MCE	Cat# HY-L025
cetuximab	MCE	Cat# HY-L025
erlotinib	MCE	Cat# HY-L025
docetaxel	MCE	Cat# HY-L025
etoposide	MCE	Cat# HY-L025
mitoxantrone	MCE	Cat# HY-L025
bortezomib	MCE	Cat# HY-L025
decitabine	MCE	Cat# HY-L025
vinorelbine	MCE	Cat# HY-L025
folinic acid	MCE	Cat# HY-L025
5-FU	MCE	Cat# HY-L025
gefitinib	MCE	Cat# HY-L025
pemetrexed	MCE	Cat# HY-L025
capecitabine	MCE	Cat# HY-L025
paraformaldehyde	Beyotime	Cat# P0099
bovine serum Albumin	Sigma-Aldrich	Cat# V900933
sucrose	Sigma-Aldrich	Cat# S7903
urea	Sigma-Aldrich	Cat# U5378
D-sorbitol	Sigma-Aldrich	Cat# S1876
glycerol	Sigma-Aldrich	Cat# G5516
DMSO	Sigma-Aldrich	Cat# D8418
Matrigel	Corning	Cat# 356237
Collagenase	Sigma	Cat# C9407
Critical Commercial Assays
CellTiter-Glo 3D cell viability assay	Promega	Cat# G9683
Deposited Data
RNA-seq	This paper	GSE161928
Whole-exome sequencing (WES)	This paper	PRJNA679439
Experimental Models: Organisms/Strains
Mouse: BALB/c	Guangdong Medical Laboratory Animal Center	NA
Software and Algorithms
Prism Graphpad 6	GraphPad Software	NA
Other
HFE-7000	3M	Cat# 200418AT
1 ml / 10 ml injection syringe	Yuekang	NA
SYLGARD 184 Silicone Elastomer Kit	Dow	Cat# 01673921
24T PTFE tubing, ID = 0.56 mm	Woer	NA
Heater	homemade	NA
Refrigerator	AUS	Cat# JC-95
3D printer	Homemade	NA
Needle, Blunt end, gauge size 22	Local supplier	NA
Injection Pump	LEAD FLUID	Cat# TYD01
Biopsy punch	Miltex	Cat# 33-31AA