Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 22;5(6):e00604-20. doi: 10.1128/mSystems.00604-20

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Ambrosi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 1 — A. baumannii binds to CEACAM receptors. Strain AB5075 grown to exponential phase was incubated with 10 µg of the indicated recombinant proteins and subjected to pulldown. (A) Pulldown samples (PD) were resolved by Tricine-PAGE and visualized by Western blotting using the anti-CEACAM antibodies (MAb 6G5j and C5-1X) and HRP-conjugated secondary antibodies. Purified proteins (50 ng) were loaded as controls (Ctrl). The recombinant CEACAM1 protein lacking the complete N-domain (CEACAM1ΔN) was also assayed. One representative experiment of four is shown. (B) Pulldown bacteria incubated with MAb 6G5j and TRITC-labeled secondary antibodies were centrifuged on polylysine-treated coverslips and mounted for bright-field (BF) and fluorescence microscopic analyses. One representative experiment of four is shown.