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Technological process of capturing single trophozoites for scRNA-seq. (A) Three groups of single trophozoites are captured using a limited dilution method and subjected to scRNA-seq. (B) Single trophozoite (arrow) in 1 μl culture medium. (C) Asexually proliferated trophozoites (arrows) in the CHO cell monocyte layer. (D) Trophozoites (arrows) in liver abscess tissue.
