Figure 1.
NETosis induction and inhibition. A, kinetics of NET formation in freshly isolated neutrophils induced with the indicated stimuli. At the indicated time points SYTOXTM Green was added to quantitate DNA release by reading the fluorescence at 532 nm. B, influence of NSP inhibitors on NET formation. Neutrophils were induced with the indicated stimuli for 4 h in the presence of 2.5 μm of the inhibitors of the indicated enzymes, individually or in combination (Combo). Released DNA was measured using SYTOXTM Green by reading at 532 nm. Each stimulus, color coded as in panel A, resulted in DNA release. A no-inhibitor control was set at 100% and other samples were normalized to this control. Values are mean ± S.D. of at least three biological replicates.