Figure 5.
Specific antibodies reveal that NSPs are bound to NETs, but in an inactive form. Images represent neutrophils induced with PMA and treated with the Cy5-labeled specific NSP probes (red), NSP-specific antibodies imaged with Alexa FluorTM 568 coupled secondaries (blue) followed by fixation and addition of SYTOXTM Green to stain DNA (green). In all cases probe staining is punctate and not associated with DNA, extracellular or intracellular. However, each NSP antigen substantially decorates DNA, revealing that NET (DNA)-associated NSPs are inactive.