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. 2020 Oct 15;295(51):17738–17751. doi: 10.1074/jbc.RA120.015534

Figure 1.

Figure 1.

ARID4B loss leads to meso/endodermal differentiation defects. a, design of the shRNA screen. b, waterfall plot of shRNAs ranked by log2 of the enrichment score in differentiated over undifferentiated cells. Negative controls are in red (not visible since their enrichment score is close to zero) and positive controls (PcG complex members) are in black. c, endoderm differentiation efficiency is plotted as % BRACHYURY-positive cells on day 5 of differentiation. Negative control: nontargeting shRNA. d, flow cytometry data for endoderm differentiation. Negative control; nontargeting shRNA. Bry-GFP, Foxa2-hCD4 mESCs were transduced with either nontargeting or Arid4b-targeting shRNAs. After differentiation toward endoderm, expression of BRACHYURY (GFP, x axis) and FOXA2 (hCD4, y axis) were determined by flow cytometry. e–l, RT-qPCR of selected transcripts during endoderm differentiation time course in WT, arid4bΔ, or arid4bΔ cells that re-express human ARID4B.