Figure 3.

Global gene expression changes upon ARID4B loss. a, RNA-seq heat map of genes related to primitive streak formation in endoderm- and mesoderm-directed WT and arid4bΔ cells. b–f, RT-qPCR of Goosecoid (b), Cxcr4 (c), Eomes (d), Aplnr (e), and Gata4 (f) transcripts during endoderm differentiation time course in WT, arid4bΔ, or arid4bΔ cells that re-express human ARID4B. g–j, RT-qPCR of Goosecoid (g), Mesp1 (h), Eomes (i), and Nodal (j) transcripts during mesoderm differentiation time course in WT, arid4bΔ, or arid4bΔ cells that re-express human ARID4B. k, biological processes down-regulated in endoderm directed arid4bΔ cells (Gene set enrichment analysis). l–o, gene set enrichment analysis (GSEA) plot of selected biological processes that are down-regulated in endoderm-directed arid4bΔ cells.