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. 2020 Dec 11;12(12):3734. doi: 10.3390/cancers12123734

Figure 3.

Figure 3

siRNA-mediated knockdown of HDAC6 caused lower cell spreading, cell proliferation, and cell migration. Cells were transfected with HDAC6 siRNA or negative scrambled control for 48 h followed by continuous measure of cell index by xCELLigence system. (A)/(i),(ii) Representative graphs from the real time xCELLigence proliferation system comparing growth profiles of scrambled siRNA treated SMG cells and HDAC6 transfected SMG cells. (A)/(iii),(iv) Cell index values taken from the xCELLigence system monitoring the cells at time intervals, observing the rate of adhesion/spreading (3 h) and proliferation (6–70 h). Wells were analyzed in duplicate, and two biological replicates were performed to validate results, n = 2. Experiments were analyzed by a two-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (A)/(i),(ii) SMG5, (A)/(iii),(iv) SMG6. (B) Migration assay with SMG5 cells (B)/(i) and SMG6 cells (B)/(ii) measured continuously as cell index using the migration xCELLigence system following siRNA transfection. Wells were analyzed in duplicate and two biological replicates were performed to validate results.