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. 2020 Dec 10;12(12):1423. doi: 10.3390/v12121423

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of TH6744 on proteome thermal stability in living cells. (A) TPP-TR and 2D-TPP experimental workflow. (B) ΔTm across the proteome of HAZV-infected SW13 cells treated with 20 μM TH6744 in TPP-TR. Number of proteins were identified as gene symbol centric. Data are from one experiment, samples carried out in biological duplicate. Colored dots indicate proteins that passed selection criteria (see Methods) and in addition have ΔTm > 2 °C. (C,D) TPP-TR melt curves of DNAJB11 (C) and or HSPA1A (D). Normalized relative protein intensities from TPP-TR were fitted using sigmoidal 4-parameter curve fitting. Data are expressed as mean ± SD from n = 2 biological replicates. (E) Distribution of relative protein abundance as Log2 fold change after indicated TH6744 treatments compared to DMSO. Data are from the 2D-TPP experiment.