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. 2020 Dec 10;12(12):1423. doi: 10.3390/v12121423

Figure 6.

Figure 6

TH6744 increases the interaction between host cell Hsp70 and HAZV NP. (A) Isothermal TH6744 dose-response CETSA at 59 °C in living U87 cells. U87 cells were treated with indicated TH6744 doses for 1 h followed by CETSA at 59 °C and Western blotting. Data of n = 1 biological replicate are shown. (B) Immunoprecipitation of endogenous Hsp70 in HAZV-infected Hek293T cells. Hek293T cells were infected with HAZV (MOI 1) for 24 h, treated with 20 μM TH6744 for 1 h and processed for immunoprecipitation with Hsp70 antibody (detecting protein product of HSPA1A gene) or IgG control. Data are representative of n = 2 biological replicates. (C) Quantification of HAZV NP signal from B, HAZV NP signal is presented as relative to Hsp70 signal. Data are expressed as mean ± SD from n = 2 biological replicates. Statistical significance was determined by using unpaired Student’s t-test.