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. 2020 Dec 10;12(12):3784. doi: 10.3390/nu12123784

Figure 2.

Figure 2

Ex vivo intestinal rat secretions induced by simultaneous stimulation by hTAS2R5 and hTAS2R39 agonist (a) and (b) or by Procyanidin B2 (c). (a) Segments of ileum were treated with epicatechin 1 mM, B2gallate 20 µM for 30 min or 1,10-Phenantroline 150 µM + Thiamine 1 mM for 45 min. (b) Segments of duodenum were treated with epicatechin 1 mM or B2gallate 20 μM for 30 min. (c) Segments of ileum (for tGLP1, vertical and squared lines) and duodenum (for CCK, dotted columns) were treated with B2 67 or 300 µM for 30 min, or B2 300 μM + epicatechin 1 mM for 45 min. Afterwards, medium was collected and respective enterohormones in the medium were quantified by ELISA (n = 6–10 segments). Mean ± SEM. (c) Results are calculated versus basal respective secretion in each hormone (Arbitrary Units, A.U.) * denotes p < 0.05 vs. control, # indicate p < 0.1 vs. control.