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. 2020 Dec 11;21(24):9433. doi: 10.3390/ijms21249433

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Histone modifications at intact L1s. ChIP-qPCR was performed in the cell lines VM-Cub-1 and 5637. (a) Expression of overall LINE-1, UID-33, UID-66 and UID-108 in VM-Cub-1 and 5637 cells as determined by qRT-PCR. ChIP was validated by qPCR for GAPDH (b), CTCFL (c) and GRM6 (d). (e)–(h) ChIP results for promoters of overall LINE-1 (e), UID-33 (f), UID-66 (g) and UID-108 (h). All ChIP results were calculated as percentages of input and the value of the respective normal IgG control (mouse or rabbit) was subtracted, which may yield negative values in cases of low enrichment. Mean values of one of two independent experiments are shown; each PCR was performed in triplicate.