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. 2020 Dec 9;8(12):587. doi: 10.3390/biomedicines8120587

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Representative images of CAE-staining with CAE⁺-cells indicated by arrows and F4/80-staining with F4/80⁺-cells stained in red (both at 400× magnification, scale bar representing 20 µm valid for all images) of livers from mice fed high fat diet (HFD), control diet (CD) or standard diet (SD); (b) Relative amount of granulocytes (CAE⁺) (HFD: n = 28; CD: n = 15, SD: n = 15); (c) Relative amount of macrophages (F4/80⁺) (HFD: n = 29; CD: n = 15, SD: n = 15). Data presented as mean ± standard deviation. Significance of differences between the groups was tested by one-way ANOVA on Ranks (Kruskal–Wallis) in (b) or ordinary one-way ANOVA in (c); **** p < 0.0001, *** p < 0.001 ** p < 0.01.