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ELISA with ligand-free rLen c 3 or with rLen c 3 preincubated with lipid ligands. The patient sera containing sIgE to this allergen were used. Differences in mean A values were compared by a Wilcoxon signed-rank test (W-test). No significant difference in IgE binding to rLen c 3 alone or to the allergen after its preincubation with different lipid ligands was observed (p value > 0.1).
