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. 2020 Dec 13;12(12):3748. doi: 10.3390/cancers12123748

Figure 7.

Figure 7

Effect of CM414 on TGFβ1 and lipopolysaccharide (LPS)-mediated activation of cholangiocytes. (a) qPCR analysis of a-SMA and collagen Iα1 (COLIα1) expression in H69 cells treated with CM414 (5 μM) for 24 h and then stimulated with TGFβ1 (5 ng/mL) for another 24 h. (b) immunoblot analyses of α-SMA, collagen-1 and α-tubulin protein levels in H69 cells treated as indicated above. (c) qPCR analyses of activin membrane-bound inhibitor (BAMBI) and PGC-1α expression in H69 cells treated as indicated above. (d) immunoblot analyses of phospho-SMAD3 and SMAD3 protein levels in H69 cells treated as indicated above. (e) qPCR analysis of MCP1 and IL6 mRNA expression in H69 cells treated with CM414 (5 μM) for 24 h and then stimulated with LPS (1 μg/mL) for another 24 h. (f) immunoblot analyses of phospho-IκBa, IκBa, phospho-p65 and p65 protein levels in H69 cells treated with CM414 (5 µM) for 24h and then stimulated with LPS (1 µg/mL for 15 min and 30 min. Representative blots of three experiments performed in duplicate are shown. * p < 0.05. ** p < 0.01.