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. 2020 Dec 12;11(12):1495. doi: 10.3390/genes11121495

Figure 2.

Figure 2

miRNAs Let-7b in EVs. (A) qPCR of pre-miRNA Let-7b and mature miRNA Let-7b in PNT2-derived EVs (PNT2-EVs) and PC3-derived EVs (PC3-EVs). Gene expression values were normalized to U6 snRNA and presented as 2−ΔΔCt. Relative RNA abundance in PNT2-EVs was assumed as 1. Data represent mean ± SD (n = 4). * p < 0.05 vs. PNT2-EVs. (B) Mature miRNA Let-7b (lane 3) and pre-miRNA Let-7b (lane 4) in PNT2-EVs, normalized for U6 (lane 2). (C) Mature miRNA Let-7b (lane 3) and pre-miRNA Let-7b (lane 4) in PC3–EVs, normalized for U6 (lane 2). (D) Comparative analysis of the expression levels of precursor and mature forms of prostate cells and their pEVs. Data represent mean ± SD (n = 4). * p < 0.05 vs. PNT2.