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Intracellular localization of EVs. TPA-differentiated THP-1 cells were exposed to 100 μg/mL DiD’-stained PC3-EVs. At the indicated time points, cells were fixed, actin filaments were stained with FITC-labeled phalloidin and nuclei were counterstained with DAPI. The images are representative of one out of three separate experiments. Magnification 63×.
