Figure 4.

Phytophthora infestans peptide-based detection in mycelium and in planta. (a) Separation of proteome profiles of two independent isolates used for the evaluation P. infestans mycelium proteome. Based on relative abundances of most abundant proteins (786 identified proteins with at least two unique peptides); (b) representative images of plant material and separation of proteome profiles of S. tuberosum leaf 72 and 96 h after inoculating with P. infestans. Presented data visualized by PCA are means and standard deviation (n = 5), different letters indicate significant differences (Kruskal–Wallis test, p < 0.05). Based on relative abundances of most abundant proteins (408 identified proteins with at least two unique peptides).