Table 2.

The experimental in vitro setup testing the analytical detection limit of bovine blood in bovine fecal matter in different titrations tested with Hemo-Fec^®. The letters A, B and C are the repeated samples made from each mixture of feces and whole blood (sub-batches), and the letters “N” and “P” represents the negative and positive samples, respectively. Batches 1 and 2 were comprised of feces from two cows whereas batch 3 and 4 were made with feces form one cow. Batch 2 and 3 were equal in both blood volumes and results, hence they were shown as one result. Likewise, the four sub-batches with 0 mL of blood added are shown as one result.

mL Blood per 1000 g of Feces [Batch Number]	Sample	Time (hours) after Mixing Feces and Blood
		0	8	20	32	44	56
0	A	N	N	N	N	N	N
	B	N	N	N	N	N	N
[1, 2, 3, 4]	C	N	N	N	N	N	N
0.25	A	N	N	N	N	N	N
	B	N	N	N	N	N	N
[4]	C	N	N	N	N	N	N
0.5	A	N	N	N	N	N	N
	B	N	N	N	N	N	N
[2, 3]	C	N	N	N	N	N	N
0.75	A	N	N	N	N	N	N
	B	N	N	N	N	N	N
[4]	C	N	N	N	N	N	N
1	A	P	N	P	N	N	N
	B	P	N	P	N	N	N
[4]	C	P	N	N	P	N	N
2	A	P	P	P	P	P	P
	B	P	P	P	P	P	P
[2, 3]	C	P	P	P	P	P	P
4.5	A	P	P	P	P	P	P
	B	P	P	P	P	P	P
[2, 3]	C	P	P	P	P	P	P
5	A	P	P	P	P	P	P
	B	P	P	P	P	P	P
[1]	C	P	P	P	P	P	P
25	A	P	P	P	P	P	P
	B	P	P	P	P	P	P
[1]	C	P	P	P	P	P	P
50	A	P	P	P	P	P	P
	B	P	P	P	P	P	P
[1]	C	P	P	P	P	P	P