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. 2020 Dec 10;21(24):9411. doi: 10.3390/ijms21249411
aa amino acids
ADCC Antibody-Dependent Cellular Cytotoxicity
AZM acetazolamide
BSA Bovine Serum Albumin
CA carbonic anhydrase
CA IX carbonic anhydrase IX
CA XII carbonic anhydrase XII
CA XIIDE3 recombinant extracellular domain of human CA XII produced in E. coli DE3 strain
CA XIIHEK recombinant extracellular domain of human CA XII expressed in human cell line HEK–293
CDR Complementarity–Determining Regions
ELISA Enzyme-Linked ImmunoSorbent Assay
FC flow cytometry
FR framework region
H Ig heavy chain
HAMA human anti-mouse antibody
HRP horseradish peroxidase
Ig immunoglobulins
IgG immunoglobulin G
Kd apparent dissociation constant
L Ig light chain
MAbs monoclonal antibodies
OD optical density
PBS-T 0.1% Tween-20 in PBS
PDB Protein Data Bank
PET positron emission tomography
PVDF polyvinylidene difluoride
RAbs recombinant antibodies
RIT radioimmunotherapy
RT room temperature
scFv single-chain fragment variable
scFv-Fc scFv fused to human IgG1 Fc fragment
SDS–PAG sodium dodecyl sulfate-polyacrylamide gel
SDS–PAGE SDS–PAG electrophoresis
TSPyV trichodysplasia spinulosa-associated polyomavirus
V variable region of Ig
VH variable region of Ig heavy chain
VL variable region of Ig light chain
VLY vaginolysin
WB Western blot