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Methods for genomic and transcriptional targeting. (A) Paired-guide RNAs recruit Cas9 to the target lncRNA gene resulting in a complete knockout. (B) SgRNAs direct Cas9 to the splice sites of lncRNA genes resulting in complete knockout. (C) A sgRNA tethers dCas9-KRAB to the TSS resulting in gene knockdown. (D) A sgRNA guides dCas9-VP64 to the TSS resulting in transcriptional activation.
