Figure 2.

Methods for direct targeting. (A) Binding of the ASO to the lncRNA transcript leads to RNase-H mediated degradation resulting in post-transcriptional knockdown, which can occur in either the nucleus or the cytoplasm. (B) A sgRNA guides Cas13 to the lncRNA transcript resulting in post-transcriptional knockdown, which can occur in either the nucleus or the cytoplasm. (C) Red arrows depict shRNA mediated RNAi in which shRNAs are integrated into the genomic DNA prior to transport to the cytoplasm. Black arrows depict siRNA mediated RNAi in the cytoplasm. RNase Dicer cleaves double stranded RNA to generate single-stranded RNAs. The single-stranded RNAs form a complex with RISC and the lncRNA, resulting in degradation of the transcript by Ago2.