Table 2.

Preparation of the calibration solutions.

Mass Concentration of Calibration Solutions
Calibration Solution	Mixed Standard Solution	Mixed ISTD Solution	NIV	DON	3-Ac DON	15-Ac DON	HT-2	T-2	ZEN
	µL	µL	µg/mL	µg/mL	µg/mL	µg/mL	µg/mL	µg/mL	µg/mL
1	25	100	0.078	0.391	0.047	0.047	0.016	0.016	0.031
2	50	100	0.156	0.781	0.094	0.094	0.031	0.031	0.063
3	100	100	0.313	1.563	0.188	0.188	0.063	0.063	0.125
4	200	100	0.625	3.125	0.375	0.375	0.125	0.125	0.250
5	400	100	1.250	6.250	0.750	0.750	0.250	0.250	0.500
6	600	100	1.875	9.375	1.125	1.125	0.375	0.375	0.750
Mass Concentration of Isotopically Labelled Analytes (µg/mL) in All Calibration Solutions
			0.313	1.563	0.188	0.188	0.063	0.063	0.125