Table 1.
Bacterial strains and plasmids used for tailoring heterologous rhamnolipid production with phenazine producing P. putida.
| Strains/Plasmids | Characteristics | Source |
|---|---|---|
| Strains | ||
| P. aeruginosa PAO1 | Wildtype | DSMZ |
| P. putida 14phz2 | PCA producer | [19] |
| P. putida RL | RL producer | this study |
| P. putida RL-PCA | PCA and RL producer | this study |
| P. putida RL-MS | PhzMS and RL producer | this study |
| P. putida RL-PYO | PCA, PYO, and RL producer | this study |
| Plasmids | ||
| pJNN.rhlAB | oriRO1600 for Pseudomonas and oriCoIE1 for E. coli; gentamycin resistance-cassette, salicylate-inducible nagR/pNagAa promoter, rhlA and rhlB genes (P. aeruginosa PAO1) | this study |
| pJNN.rhlAB.phzMS | oriRO1600 for Pseudomonas and oriCoIE1 for E. coli; gentamycin resistance-cassette, salicylate-inducible nagR/pNagAa promoter, phzM, phzS, rhlA and rhlB genes (P. aeruginosa PAO1) | this study |
| pBNT.14phz2 | ORI: ori/IHF for replication in E. coli and Pseudomonas; kanamycin resistance-cassette, salicylate-inducible nagR/pNagAa promoter, phzA2-G2 genes (P. aeruginosa PA14) | [19] |
| pSK02 | oriR6K-pir dependent origin of replication; oriI -origin of transfer; riboJ gene; kanamycin resistance-cassette, rhlA and rhlB genes (P. aeruginosa PAO1) | [16] |