Table 2.
Recombinant rhamnolipid production in batch experiments in this study compared to other Pseudomonas producers.
| Organism | Medium | Substrate (conc. in g/L) | Duration | Maximal RL Titer (g/L) | Growth Condition | Ref. |
|---|---|---|---|---|---|---|
|
P. putida KT42C1 pVLT31_rhlAB |
LB | Glucose (10) | 42 h | 1.5 | Flask, aerobic | [11] |
|
P. putida KT2440 pSynPro8 |
LB | Glucose (10) | 22 h | 3.22 | Flask, aerobic | [10] |
| P. putida KT2440 * pSynPro8oT | ModR | Glucose (253) | 72 h | 14.9 | Benchtop bioreactor, aerobic | [38] |
| P. putida KT2440 Δflag SK4 | Delft | Glucose (11) | 10 h | 1.48 | Benchtop bioreactor, aerobic | [16] |
| P. putida RL KT2440 pJNN.rhlAB | LB | Glucose (10) | 30 h | 0.73 | Microscale, aerobic | this study |
| P. putida RL KT2440 pJNN.rhlAB | Delft | Glucose (10) | 30 h 72 h |
0.36 0.11 |
Microscale, aerobic Flask, aerobic |
this study |
|
P. putida RL-PCA KT2440 pJNN.rhlAB_pBNT.14phz2 |
Delft | Glucose (10) | 30 h 72 h |
0.11 0.15 |
Microscale, aerobic Flask, aerobic |
this study |
| P. putida RL-PCA KT2440 pJNN.rhlAB_pBNT.14phz2 | Delft | Glucose (5) | 10 d | 0.02 | Benchtop BES, PA ** | this study |
| P. putida RL-PCA KT2440 pJNN.rhlAB_ pBNT.14phz2 | Delft | Glucose (5) | 25 d | 0.03 | Electrobioreactor BES, PA ** | this study |
* Data in this row represent the highest titer achieved with recombinant P. putida. However, this process was operated in fed-batch with a continuous glucose feed and does not allow for direct comparison with other presented data from batch operations. ** PA: passive aeration via open vent filters.