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. 2020 Dec 12;8(12):1977. doi: 10.3390/microorganisms8121977

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phylogeny of the tuf gene region used in this study. (A) Phylogeny based on tuf genes from >9000 staphylococcal genomes with tuf gene ASVs in each staphylococcal species shown in parenthesis. (B) Amplicon sequencing of tuf genes from a staphylococcal mock community composed of equal amounts of genomic DNA from Staphylococcus aureus, Staphylococcus capitis, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus lugdunensis, and Staphylococcus pseudintermedius in ≈100,000 copies/organism (left) diluted down to ≈1 copy/organism (right). Error bars indicate highest and lowest observation in each technical triplicate experiment.