Figure 5.

Protective effect of different phytochemicals on m-aconitase activity treated with 250 μM H₂O₂. Measurements were carried out as described in Methods by incubating m-aconitase (250 μg) for 10 min with different phytochemicals applied at 150 μM (75 μM in the case of resveratrol) and for 15 min with H₂O₂. Activity values are expressed as a percentage of the control (i.e., the reaction measured at time 0 in the absence of both H₂O₂ and phytochemicals) and reported as the mean value ± SD (n = 3). Means with no lowercase letter a–d in common are statistically different at a 0.05 p level of significance, according to the post hoc Duncan’s test, following ANOVA. Means with at least one common lowercase letter are not significantly different at p ≤ 0.05. In this set of experiments, m-aconitase activity under control conditions was equal to 18 ± 1 (SD, n = 3) nmol·min⁻¹·mg⁻¹ of protein.