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. 2020 Dec 11;9(12):1758. doi: 10.3390/plants9121758

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pollinia and pollen tubes of Acianthera johannensis (A,B,E,F) and A. fabiobarrosii (C,D) under light microscopy, in transverse (A–D) and longitudinal (E,F) sections. (A) Two pollinia in the stigmatic cavity. (B) Pollen grain in tetrads. (C) Pollen grain after cross-pollination. Notice the generative cell (black arrow) and the nucleus of the vegetative cell (white arrow). (D) Pollen tubes after cross-pollination in the ovary 27 days after pollination (DAP). Notice the two cells in the microgametophyte, a generative cell (black arrow), and the nucleus of the vegetative cell (white arrow). (E,F) Pollen tube in the micropyle 40 DAP in two sequential focus planes. Notice the nucleus of the vegetative cell in both figures (white arrow) and two sperm cells (black arrows), with one visible in each figure. mi, micropyle. po, pollinia. pt, pollen tube. st, stigmatic cavity.