Pollen grain germination and pollen tube growth in flowers and fruits of Acianthera fabiobarrosii (A,C,E) and A. johannensis (B,D,F) observed with an epifluorescence microscope after cross-pollination. (A,B) Pollen tubes growing in the stylar canal inside the column (7 days after pollination-DAP). In B, observe the callose plugs regularly arranged along the length of the tube (arrows). (C) Pollen tubes reaching the ovary (10 DAP). (D) Pollen tubes in the seed chamber (38 DAP). (E) General view of pollen tubes in the column and seed chamber in a fruit (120 DAP). (F) Pollen tubes near the seeds with embryo in mature fruit (90 DAP). co, column. pt, pollen tube. sc, seed chamber. se, seed. st, stigmatic cavity.