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. 2020 Dec 11;9(12):1758. doi: 10.3390/plants9121758

Figure 3.

Figure 3

Pollen grain germination and pollen tube growth in flowers and fruits of Acianthera fabiobarrosii (A,C) and A. johannensis (B,D–F) observed with an epifluorescence microscope after self-pollination. (A) Pollinia in the stigmatic cavity (five days after pollination-DAP). (B) Pollen tubes (arrow) that stopped growing near the apex of the stylar canal inside the column (7 DAP). (C) Pollen tubes with a tortuous appearance (arrows) stopped growing in the column (7 DAP). (D) Detail of the incompatible pollen tube in the column. Observe the callose plug (black arrow) and the dilated tip (white arrow). (E) Pollen tubes in a withered self-pollinated flower from a partially incompatible individual (7 DAP). Note the synchronous growth of pollen tubes in the stylar canal. (F) Pollen tubes with regular morphology in mature fruit (90 DAP). Observe some pollen tubes with irregular patterns (arrows). po, pollinia. pt, pollen tube. sc, seed chamber. st, stigmatic cavity.