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ELISA assay to determine the presence of human IL-IRA in control (media only) and cell-free stem cell-derived extract (CCM). Significantly high levels (p < 0.01) of IL-1RA identified in CCM compared to no detectable IL-1RA in control. Data represent mean + SEM. CCM was produced in two lots in duplicate from two vials and ELISA for IL-1RA was performed using CCM from each lot three separate times in triplicate.
