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. 2020 Oct 8;9(20):e017477. doi: 10.1161/JAHA.120.017477

Figure 1. Isoflurane and endothelial nitric oxide synthase protein expression with and without SAH.

Figure 1

A, Wild‐type mice were exposed to air or 2% isoflurane for 1 hour and cortical tissue harvested immediately and at 12, 36, 48, and 72 hours after isoflurane exposure were subjected to Western blot. Data indicate mean±SEM. *P<0.05, **P<0.01 vs control (no isoflurane) by t test, n=4 mice per group. B, Representative immunoblot images. C, Wild‐type mice underwent sham or SAH surgery and exposed to isoflurane 2% for 1 hour after 1‐hour post‐SAH. Cortical tissue harvested at 24 hours post‐surgery were subjected to Western blot. Data indicate mean±SEM. *P<0.05 sham vs SAH‐post‐conditioning, *P<0.05 SAH vs SAH‐post‐conditioning by ANOVA followed by Newman‐Keuls multiple comparison test (sham, n=9; SAH, n=9; SAH+Isoflurane, n=14). eNOS indicates endothelial nitric oxide synthase; postC, post‐conditioning; and SAH, subarachnoid hemorrhage.