Skip to main content
. 2020 Oct 8;9(20):e017477. doi: 10.1161/JAHA.120.017477

Figure 2. Isoflurane conditioning attenuates SAH‐induced vasospasm in wild‐type mice.

Figure 2

A, Mice underwent sham or SAH surgery and exposed to isoflurane 2% for 1 hour after 1‐hour post‐SAH. On post‐surgery day 3, mice were perfused with ROX‐SE staining and vessel diameter in the proximal middle cerebral artery ipsilateral to suture perforation was measured. Data indicate mean±SEM. B, Representative images of 5‐(and‐6)‐Carboxy‐X‐rhodamine, succinimidyl ester casted vessels. Arrow mark represents the middle cerebral artery vessel. Scale bar=500 μm. C, Neuroscore was assessed daily and until 3 days after SAH. Data indicate mean±SEM. MCA indicates middle cerebral artery; postC, post‐conditioning; ROX‐SE, 5‐(and‐6)‐Carboxy‐X‐rhodamine, succinimidyl ester; SAH, subarachnoid hemorrhage; and WT, wild type. *P<0.05 sham vs SAH, **P<0.01 SAH vs SAH‐post‐conditioning, by ANOVA and Newman‐Keuls multiple comparison test, # P<0.05 SAH‐post‐conditioning vs SAH by 2‐way repeated measures ANOVA followed by Newman‐Keuls multiple comparison test.