Table 1.

Biochemical Analyses of sRBC Diluted to 10% Volume in KH‐Buffered Media

Parameter (mmol/L)	Days of Storage
	KH Media	7	30–35	40	50
pH	7.38±0.01	7.40±0.01	7.41±0.01	7.40±0.003	7.41±0.01
Osmolarity, mOsm/L	315±0.58	314±2.65*	315±0.86	318±1.86	319±1.67
K+	4.48±0.04	5.82±0.16*	9.28±0.41*,**	9.28±0.33*,**	9.65±0.26*,**
Lactate	0.30±0.002	0.79±0.96	2.76±0.24*,**	2.42±0.17*,**	2.98±0.20*,**
Cl−	117.9±0.51	117.9±0.23	117.6±0.32	116.7±0.61	119.3±0.33
Ca2+	1.70±0.05	1.14±0.11*	0.97±0.10*	1.10±0.13*	1.27±0.11*
Na+	151±0.32	149±0.34*	145±0.45*,**	145±0.80*,**	147±0.33*,**

Storage age was associated with deviations in the electrolyte composition of sRBC samples. Mean±SEM. KH indicates Krebs‐Henseleit media; and sRBC, supernatant from red blood cell units.

*Value differs significantly from control KH buffer, or ^**Value differs from day 7 sample (P<0.05). n=3–10 per group.